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. Author manuscript; available in PMC: 2016 Apr 12.
Published in final edited form as: Biochem J. 2015 Apr 27;469(1):71–82. doi: 10.1042/BJ20141455

Figure 1. Lipid accumulation and insulin signalling in liver.

Figure 1

AdKO mice fed either regular chow or on a 60 % HFD for 6 weeks. HFD mice received an additional 2-week adiponectin or saline treatment at the dosage of 3 μg/g of body weight via intraperitoneal injection. Liver tissues were then collected 15 min after 4 units/kg insulin stimulation for later analysis. (A) Haematoxylin and eosin staining of paraffin-embedded cross-sections of liver tissues (×200). (B) Oil Red O staining of cryosections of liver tissues (×200). (C) Triacylglycerol content in liver tissues. (D and E) Representative Western blot and analysis of insulin-stimulated Akt at phosphorylation sites Ser473 and Thr308. Scale bars, 200 μm. Results are means±S.E.M. HF, HFD; Ad, adiponectin; fAd, full-length adiponectin; Tg, triacylglycerol. *Significant difference between chow and HFD group. ∧Significant difference between saline-treated and adiponectin-treated HFD groups. *∧P < 0.05, **∧∧P < 0.01.