Table 2.

Methylation-sensitive amplified polymorphism analysis of cytosine methylation patterns in the extracted B. napus.

EcoRI Digest (isoschizomer) lane∗				Methylation pattern in		Subtype of methylation
HpaII	MspI	HpaII	MspI	B. napus	Addition Lines
–	–	–▾	+▴	#CCGG GGCC	CCGG GGCC	B3
–	–	+	–	CCGG GGCC	CCGG GGCC	B4
–	+	+	+	CCGG GGCC	CCGG GGCC	B1
+	–	+	+	CCGG GGCC	CCGG GGCC	B2
+	+	–	+	CCGG GGCC	CCGG GGCC	A2
+	+	+	–	CCGG GGCC	CCGG GGCC	A1
–	+	–	–	CCGG GGCC	CCGG GGCC	A3
+	–	–	–	CCGG GGCC	CCGG GGCC	A4
–	+	+	–	CCGG GGCC	CCGG GGCC	C1
+	–	–	+	CCGG GGCC	CCGG GGCC	C2
+	+	+	+	CCGG GGCC	CCGG GGCC	D1
+	–	+	–	CCGG GGCC	CCGG GGCC	D2
–	+	–	+	CCGG GGCC	CCGG GGCC	D3

#The combination of four letter represents the specific restriction endonuclease sequence and the underscored letter represent the restriction endonuclease digestion site. ▴Represented presence (+) of a band resulted from the EcoRI/HpaII-digested^∗ or EcoRI/MspI-digested^∗ genomic DNA of parental B. napus and nine additions. ▾ Represented absence (-) of a band resulted from the EcoRI/HpaII-digested^∗ or EcoRI/MspI -digested^∗ genomic DNA of parental B. napus and nine additions. ++,+-, -+ and -- represented CCGG/GGCC or C5^mCGG/GGCC, 5^mCCGG/GGCC, C5^mCGG/GGC5^mC and 5^mCCGG/GGCC5^m, respectively.