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Illustration of the quantitation workflow using the L/H ratios of endogenous plant-derived light peptides (L) and the QconCAT-derived isotope labeled standard peptides (H). In the spiking experiments, it will be important that the amount of the spiked-in standard (H, heavy) needs to be added in the same amount in all experiments/conditions to be compared. This is indicated by equal peak intensities in the illustrations.
