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. 2016 Apr 12;7:475. doi: 10.3389/fpls.2016.00475

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Copyright © 2016 Iqbal, Sattar and Shafiq.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Nucleotide sequence alignments of CABs (A) and their associated DNA-satellites (B,C). All the sequences were retrieved from NCBI GenBank database and aligned using ClustalW in Mega6. The isolate descriptors and accession numbers are given on the left side of each alignment. All the possible sgRNAs are summarized in the Supplementary Table S1. Only the recommended sgRNA sequence is given at the top of each alignment as black text. The protospacer adjacent motif (PAM) sequence is indicated in red text. Any mismatch outside of the seed sequence is highlighted as white text with black background.