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. 2016 Mar 9;147:847–855. doi: 10.1007/s00706-016-1713-y

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — DNA adduct formation by BaP activated with microsomes isolated from livers of control rats (a) or rats pretreated with Sudan I (b) or BaP (c) in the presence of either NADPH or NADH. Comparison with previous ³²P-postlabeling studies [15, 29] showed that adduct spot 1 has similar chromatographic properties on PEI-cellulose TLC to a guanine adduct derived from reaction with 9-hydroxy-BaP-4,5-oxide [15]. Adduct 2 is dG-N ²-BPDE adduct. For all panels, values represent mean total relative adduct labeling (RAL) ± SD (n = 3; analyses of three independent in vitro incubations)