Figure 7.

Effects of supernatants from jacalin-stimulated macrophages on HT-29 and MCF-7 cell viability. HT-29 or MCF-7 cells were incubated during 72 h with supernatants from macrophages stimulated with jacalin (2.5 to 40 μg/mL) or LPS (1 μg/mL) for 24 to 72 h. After that, MTT solution was added to the cells and four hours later the solvent solution was added (a and b), or cells were double stained with Annexin V-FITC and PI and analyzed by flow cytometry (c). Values represent the mean ± SEM of three independent experiments. ^∗ p ≤ 0,05, ^∗∗ p ≤ 0,01, and ^∗∗∗ p ≤ 0,001 compared to control.