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. 2016 Apr 12;6:24082. doi: 10.1038/srep24082

Figure 2. RNAi effects on transcript and protein abundance, and SOD activity in P. cochleariae.

Figure 2

(A) Transcript abundance of PcSOD3.1 after injection with 200 ng PcSOD3.1-dsRNA or gfp as a control, respectively. RNA from whole larvae has been used as template. The efficiency corrected Cq values of PcSOD3.1-silenced samples have been normalized to the reference genes PcRPL6 and PcRPS3 (n = 3). (B) Resulting transcript abundance of whole female adult beetles eight weeks after maturation, which were injected with PcSOD3.1-dsRNA or gfp-dsRNA during different developmental stages (n = 5). L200, larvae injected with 200 ng dsRNA; L500, larvae injected with 500 ng dsRNA; P500, pupae injected with 500 ng dsRNA; P1K, pupae injected with 1 μg dsRNA. (C) Coomassie stained SDS-PAGE with 0.3 μl of larval defence secretions from each experimental group, nine days after RNAi induction as described in (A). Here we show a cropped gel section. The full-length gel is presented in Supplementary Fig. S16. NIC, Non-injected control. (D) Time series of SOD activity of defence secretions, quantitatively determined by using a colorimetric microtiter plate assay. From pooled individuals from each experimental group 0.3 μl of defensive secretions was used in a 20 μl reaction mixture for the SOD assays. Values of the PcSOD3.1(−)-group were normalized to gfp-control (n = 3).