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. 2016 Apr 12;6:23978. doi: 10.1038/srep23978

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Mass spectrometry analysis of ExtaPEG-harvested protein from HeLa cells identified 519 unique proteins. Approximately 97% (499 of 517) of ExtraPEG-identified proteins were found in the Vesiclepedia database of described human extracellular vesicle proteins. Additionally, 95% (492 of 517) of the proteins were previously characterized as exosome proteins. Of the 519 mass spectrometry identifications, 517 were used for this analysis as there were two sets of proteins for which a single gene name was used by the Exocarta and Vesiclepedia databases to represent multiple protein accession numbers (i.e.: HLA-A represented 1A68_HUMAN and 1A69_HUMAN; HLA-B represented 1B15_HUMAN and 1B55_HUMAN).