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. 2016 Apr 12;6:24298. doi: 10.1038/srep24298

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In vitro confocal fluorescent images of the two-component delivery strategy in HER2(+) BT-474 cells showing (A) cell surface labeling by Tz(TCO)₆(AF-488)₄ followed by the co-localization of Alb(Peg₄-Tt)₁₅(Rhod)₄ after 15 min incubation at 20 °C (Scale bar: 100 μm), and (B) rapid cellular internalization after incubation at 37 °C for 4 h (Scale bar: 50 μm). (i) Full field-of-view 63x confocal images, and (ii) a zoomed-in, high-resolution section of the images (Scale bar: 20 μm).