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. 2016 Apr 12;10:39. doi: 10.3389/fnana.2016.00039

Figure 5.

Figure 5

The number of serotonin neurons is unchanged within the dorsal and median regions of the raphe nucleus in VGLUT3 KO mice. (A) Photomicrographs taken at 20× and 60× magnifications, showing SERT mRNA in both dorsal and median raphe using fluorescent in situ hybridization for SERT in VGLUT3 WT and KO mice. (B) Bar graphs illustrating the number of SERT- mRNA-containing neurons in the dorsal and median raphe of VGLUT3 WT and KO mice. (C) Photomicrographs taken at 20× (left panels) and 60× (right panels) magnifications, showing 5-HT-immunoreactive neurons in both the dorsal and median raphe, revealed by DAB staining, in VGLUT3 WT and KO mice. (D) Bar graphs illustrating the number of 5-HT neurons in the dorsal and median raphe of VGLUT3 WT and KO mice following unbiased stereological counting. Deletion of the VGLUT3 gene did not affect the number of 5-HT neurons in the dorsal and median sub-regions of the raphe.