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. 2016 Apr 12;6:24325. doi: 10.1038/srep24325

Figure 1. Production of superoxide and conidia of Coniothyrium minitans CmNox1 and CmNox2 mutants.

Figure 1

(a) Detection of superoxide using colony staining with NBT solution (0.05% w/v, 30 min) of cultures of C. minitans wild-type strain ZS-1, CmNox1 deletion mutant ΔCmNox1-6, CmNox2 deletion mutant ΔCmNox2-323 on PDA. (b) Pycnidial and conidial production of C. minitans wild-type strain ZS-1, CmNox1 deletion mutant ΔCmNox1-6, complementary transformant CmNox1-C8, and CmNox2 deletion mutant ΔCmNox2-323 12 days after incubation on PDA. Compared to ZS-1, CmNox1-C8 and ΔCmNox2-323, ΔCmNox1-6 lost the ability to produce pycnidia.