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. 2016 Apr 12;6:24186. doi: 10.1038/srep24186

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Copyright © 2016, Macmillan Publishers Limited

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Matrix optimization by monitoring LDH response to isothermal vitrification at the following conditions: Without any excipient, with each individual excipient at various concentrations, and with all excipients present at the determined optimum concentrations. All reported values are relative to the fresh control (without added excipients) set at 100%. Error bars represent standard deviation from at least three independent experiments. Connecting bars show excipient concentrations (A–E) that result in post-rehydration recovery that is not significantly (ns) different from the fresh, un-treated control. The lowest excipient concentration that provides the highest stability was used in the final matrix (F).