Figure 2. Spatial Filtering-Based Background Removal and Multi-Channel Forest Classifiers-Based 3D (SMART 3D) Reconstruction of Metastatic Heterogeneity.

(a) Schematic of the background removal process. Arrow points to fine astrocyte processes preserved by this method. (b) Schematic of multi-channel feature extraction. (c) Schematic of the process of voxel-level segmentation based on random forest classification. (d) Image of DAPI stained MDA-MB-231.Br brain metastases sample before (top left) and after (top right) DAPI cluster-based tumor segmentation and surface generation. Color codes represent individually identified tumors. Bottom panel: whole spectrum of individual morphologically heterogeneous tumors. (e) Image of DAPI (blue) and K8 (green) stained PNA.Met1 brain metastases before (top left) and after (top right) K8/DAPI-based segmentation and surface generation. Bottom panel: whole spectrum of individual morphologically heterogeneous tumors.