Fig. 6.

mRNAs for inflammatory chemokines and cytokines required for Th17 cell expansion are increased in CXCR3^−/− mice during EAE. WT (n = 5) and CXCR3^−/− (n = 5) mice were immunized with MOG. Spinal cords were collected on days 0, 10, and 14 post-immunization, and total RNA was extracted and subjected to real-time PCR analysis to determine the mRNA levels for cytokines (a) and chemokines (b). mRNA levels of target genes, normalized to the level of GAPDH, were analyzed using the ΔΔCt method. n stands for the number of mice. *P < 0.05; **P < 0.01