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. 2016 Apr 12;9:216. doi: 10.1186/s13104-016-1902-0

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© Becerra et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Schematic showing the modified Gram stain procedure. The traditional Gram stain procedure is completed in either case (top box). The major difference in the modified Gram stain procedure occurs during the dehydration process and is the application of alcoholic saffron (lower right box, highlighted in red). The yellow contrast in the connective tissue (collagen) within skin tissue is seen in the low magnification images on the bottom (Scale bar 1 mm)