Fig. 2.
Expression and activity data suggests principal limitation is not heterologous O-methyltransferase. a SDS-PAGE gel suggesting robust OMTHs expression in soluble fractions of cells sampled from flask cultures. b In vitro specific activity measurements normalized to activity at 12 h time point. Activity data indicates that OMTHs activity declines from 12 to 48 h. However, notable activity even at 48 h strongly suggests that loss of OMTHs activity is not responsible for observed reduction in rate of vanillate formation