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. 2016 Apr 11;15:61. doi: 10.1186/s12934-016-0459-x

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© Kunjapur et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of metJ deletion on protocatechuate and vanillate titers (a) in different host strains and (b) in the presence of amino acid supplementation. For these experiments, the following genes were overexpressed: aroG*,asbF _Bt, OMT _Hs , ppsA, and tktA. For the amino acid supplementation experiment (c), 10 mM of amino acid was added at induction. d Effect of overexpressing feedback-desensitized variants of metA and cysE along with aroG*,asbF _Bt, OMT _Hs , ppsA, and tktA in the RARE ∆metJ host. The control represents co-transformation with an empty pCOLADuet-1 plasmid