Fig. 5.

Orthogonal strategy of increasing S-adenosylhomocysteine (SAH) recycling through overexpression of mtn and luxS also improves vanillate titer. a The activated methyl cycle in E. coli (in black and red), along with an alternative SAH recycling route featuring a heterologous SAH hydrolase (sahH _Sc, in blue). Native genes targeted for overexpression (mtn and luxS) are shown in red. b Effect of expressing sahH _Sc with aroG*,asbF _Bt, OMT _Hs , ppsA, and tktA in the RARE ∆metJ host on protocatechuate and vanillate titers. c Effect of overexpressing mtn and luxS with aroG*,asbF _Bt, OMT _Hs , ppsA, and tktA in the RARE ∆metJ host on protocatechuate and vanillate titers. d Effect of 10 mM methionine supplementation at induction on protocatechuate and vanillate titers using strains expressing sahH _Sc. e Effect of 10 mM methionine supplementation at induction on protocatechuate and vanillate titers using strains overexpressing mtn and luxS