Table 1.
Summary of titers from key experiments
| Number | Figure | Host | Genes overexpressed | Methionine added (mM) | Protocatechuate (P) Titer (mg/L) | Protocatechuate (P) Titer (mM) | Vanillate (V) Titer (mg/L) | Vanillate (V) Titer (mM) | Cumulative P+V Titer (mM) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 1B | PTS-glu + RARE’ | aroG*, asbF, OMT | 0 | 216 | 1.4 | 135 | 0.8 | 2.2 |
| 2 | 1B | PTS-glu + RARE’ | aroG*, asbF, OMT, ppsA, tktA | 0 | 308 | 2.0 | 101 | 0.6 | 2.6 |
| 3 | 3B | PTS-glu + RARE’ | aroG*, asbF, OMT, ppsA, tktA | 0 | 323 | 2.1 | 101 | 0.6 | 2.7 |
| 4 | 4D | RARE ∆metJ | aroG*, asbF, OMT, ppsA, tktA | 0 | 262 | 1.7 | 202 | 1.2 | 2.9 |
| 5 | 4D | RARE ∆ metJ | aroG*, asbF, OMT, ppsA, tktA, metA, cysE | 0 | 185 | 1.2 | 269 | 1.6 | 2.8 |
| 6 | 5B | RARE ∆metJ | aroG*, asbF, OMT, ppsA, tktA, sahH | 0 | 231 | 1.5 | 168 | 1.0 | 2.5 |
| 7 | 5C | RARE ∆ metJ | aroG*, asbF, OMT, ppsA, tktA, mtn, luxS | 0 | 277 | 1.8 | 252 | 1.5 | 3.3 |
| 8 | 3B | PTS-glu + RARE’ | aroG*, asbF, OMT, ppsA, tktA | 10 | 246 | 1.6 | 168 | 1.0 | 2.6 |
| 9* | 4C | RARE ∆metJ | aroG*, asbF, OMT, ppsA, tktA | 10 | 200 | 1.3 | 303 | 1.8 | 3.1 |
| 10 | 5D | RARE ∆metJ | aroG*, asbF, OMT, ppsA, tktA, sahH | 10 | 154 | 1.0 | 269 | 1.6 | 2.6 |
| 11 | 5E | RARE ∆ metJ | aroG*, asbF, OMT, ppsA, tktA, mtn, luxS | 10 | 123 | 0.8 | 370 | 2.2 | 3.0 |
| 12 | S3 | RARE ∆ metJ | aroG*, asbF, OMT, ppsA, tktA, metA, cysE | 20 | 139 | 0.9 | 420 | 2.5 | 3.4 |
Rows in bold formatting are highlighted in “Discussion” section
* Titers shown for experiment 9 correspond to samples obtained 48 h after induction, which was the final sampling time. All other titers correspond to samples obtained 72 h after induction