Table 2.
Strains and plasmids used in this study
| Name | Relevant genotype | Source |
|---|---|---|
| E. coli strains | ||
| DH5α | F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Invitrogen |
| DH10B | F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ− rpsL nupG | Invitrogen |
| MG1655 | F− λ− ilvG- rfb-50 rph-1 | ATCC 700926 |
| MG1655(DE3) | F− λ− ilvG- rfb-50 rph-1 (DE3) | Ref. [55] |
| RARE | MG1655(DE3) ∆dkgB ∆yeaE ∆(yqhC-dkgA) ∆yahK ∆yjgB | Ref. [5] |
| RARE ∆metJ | MG1655(DE3) ∆dkgB ∆yeaE ∆(yqhC-dkgA) ∆yahK ∆yjgB ∆metJ | This study |
| PTS− glu+ | MG1655(DE3) ∆ptsHIcrr Pglk::Pcon* galPq | This study, but based on Ref. [56] |
| PTS− glu+ RARE’ | MG1655(DE3) ∆ptsHIcrr Pglk::Pcon* galPq ∆(yqhC-dkgA) ∆yahK ∆yjgB | This study |
| PTS− glu+ RARE’ ∆metJ | MG1655(DE3) ∆ptsHIcrr Pglk::Pcon* galPq ∆(yqhC-dkgA) ∆yahK ∆yjgB ∆metJ | This study |
| E. coli plasmids | ||
| pCP20 | λ cI857 (ts), λ pr Repts, AmpR, CmR, λ pr FLP | CGSC 7629 |
| pKD13 | oriRγ, AmpR, kan | CGSC 7633 |
| pKD46 | oriR101, repA101 ts, AmpR, araC, araBp-λ γ-λ β-λexo | CGSC 7739 |
| pETDuet-1 | AmpR, lacI, T7lac | Novagen |
| pACYCDuet-1 | CmR, lacI, T7lac | Novagen |
| pCOLADuet-1 | KanR, lacI, T7lac | Novagen |
| pCDFDuet-1 | StrR, lacI, T7lac | Novagen |
| pACYC-car-sfp | pACYCDuet-1 harboring car opt (carboxylic acid reductase from Nocardia iowensis, codon-optimized for expression in E. coli) and sfp opt (phosphopantetheinyl transferase from Bacillus subtilis, codon optimized for expression in E. coli) | Ref. [5] |
| pET-OMT-asbF | pETDuet-1 harboring Hs-S-COMT opt (catechol O-methyltransferase from Homo sapiens, codon-optimized for expression in E. coli) and asbF opt (dehydroshikimate dehydratase from Bacillus thuringiensis, codon optimized for expression in E. coli) | Ref. [5] |
| pS4 | Plasmid containing the shikimate module, version 4, kindly provided by the Keasling Lab at UC Berkeley. (Source of aroG*-ppsA-tktA artificial operon) | Ref. [57] |
| pACYC-aroG* | pACYCDuet-1 harboring the feedback-resistant aroG* from E. coli | This study |
| pACYC-aroG*-ppsA-tktA | pACYCDuet-1 harboring three E. coli genes in an artificial operon: aroG*, ppsA, and tktA | This study |
| pCOLA-metK | pCOLADuet-1 harboring the E. coli metK gene | This study |
| pET-OMT | pETDuet-1 harboring Hs-S-COMT opt | This study |
| pCOLA-metA* | pCOLADuet-1 harboring a feedback-desensitized version of E. coli metA (27_Arg- > Cys, 296_Ile- > Ser, and 298_Pro- > Leu, “metA*”) | This study |
| pCOLA-cysE* | pCOLADuet-1 harboring a feedback-desensitized version of E. coli cysE (95_Val- > Arg, and 96_Asp- > Pro, “cysE*”) | This study |
| pCOLA-metA*-cysE* | pCOLADuet-1 harboring an artificial operon consisting of the metA* and cysE* genes | This study |
| pCDF-car-sfp | pCDFDuet-1 harboring an artificial operon containing car opt and sfp opt | This study |
| pCOLA-mtn | pCOLADuet-1 harboring the E. coli mtn gene | This study |
| pCOLA-mtn-luxS | pCOLADuet-1 harboring an artificial operon consisting of the E. coli mtn and luxS genes | This study |
| pCOLA-sahH | pCOLADuet-1 harboring sahH Sc (S-adenosylhomocysteine hydrolase from S. cerevisiae, codon-optimized for expression in E. coli) | This study |