Table 3.

Synthesized gene sequences used in this study

Gene (and restriction enzymes)	DNA sequence. Restriction enzyme sites are underlined and start/stop codons are in bold
metA* (NdeI/AatII)	AAAAAACAT ATGCCGATTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTTGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGAGCACGCTATACGATCTACGGCACATGAATCCAACGCTGGATTAA GACGTCAAAAAA
cysE* (AatII/XhoI)	AAAAAAGACGTCTAATAAAAGGAGATATACCATGTCGTGTGAAGAACTGGAAATTGTCTGGAACAATATTAAAGCCGAAGCCAGAACGCTGGCGGACTGTGAGCCAATGCTGGCCAGTTTTTACCACGCGACGCTACTCAAGCACGAAAACCTTGGCAGTGCACTGAGCTACATGCTGGCGAACAAGCTGTCATCGCCAATTATGCCTGCTATTGCTATCCGTGAAGTGGTGGAAGAAGCCTACGCCGCTGACCCGGAAATGATCGCCTCTGCGGCCTGTGATATTCAGGCGGTGCGTACCCGCGACCCGGCAAGACCCAAATACTCAACCCCGTTGTTATACCTGAAGGGTTTTCATGCCTTGCAGGCCTATCGCATCGGTCACTGGTTGTGGAATCAGGGGCGTCGCGCACTGGCAATCTTTCTGCAAAACCAGGTTTCTGTGACGTTCCAGGTCGATATTCACCCGGCAGCAAAAATTGGTCGCGGTATCATGCTTGACCACGCGACAGGCATCGTCGTTGGTGAAACGGCGGTGATTGAAAACGACGTATCGATTCTGCAATCTGTGACGCTTGGCGGTACGGGTAAATCTGGTGGTGACCGTCACCCGAAAATTCGTGAAGGTGTGATGATTGGCGCGGGCGCGAAAATCCTCGGCAATATTGAAGTTGGGCGCGGCGCGAAGATTGGCGCAGGTTCCGTGGTGCTGCAACCGGTGCCGCCGCATACCACCGCCGCTGGCGTTCCGGCTCGTATTGTCGGTAAACCAGACAGCGATAAGCCATCAATGGATATGGACCAGCATTTCAACGGTATTAACCATACATTTGAGTATGGGGATGGGATCTAA CTCGAGAAAAAA
sahH Sc (NcoI/NotI)	ATATATCC ATGGGCGCCCCTGCTCAGAATTATAAAATTGCAGATATCTCACTGGCAGCGTTCGGTCGTAAGGAAATTGAATTGGCAGAGCACGAAATGCCAGGCCTGATGGCAATTCGTAAAGCCTATGGCGACGTTCAACCGCTGAAGGGCGCACGTATCGCGGGTTGCTTACACATGACGATCCAAACCGCCGTGCTGATTGAGACATTAGTCGCTTTGGGAGCCGAGGTTACGTGGTCGTCATGCAATATCTACTCGACGCAAGATCATGCGGCAGCGGCAATCGCTGCAAGCGGCGTGCCTGTGTTTGCTTGGAAAGGTGAAACCGAAGAGGAATACCTGTGGTGCATTGAACAGCAGCTGTTTGCATTCAAGGATAACAAAAAATTGAACCTGATTTTGGACGACGGCGGGGATTTAACAACCTTAGTTCACGAGAAACATCCTGAAATGCTGGAGGATTGTTTTGGCCTTTCCGAAGAAACCACCACCGGTGTCCACCACCTCTACCGCATGGTTAAAGAGGGTAAACTTAAAGTCCCGGCTATCAATGTGAACGACTCAGTAACCAAATCCAAATTTGATAACTTATATGGTTGCCGTGAAAGCCTGGTTGACGGGATTAAGCGCGCCACGGATGTTATGCTTGCCGGAAAGGTGGCTGTTGTGGCCGGTTACGGTGACGTGGGTAAAGGATGCGCAGCCGCGTTACGTGGCATGGGTGCACGCGTGTTAGTTACGGAAATTGATCCTATCAATGCCCTGCAGGCCGCGATGGAAGGCTATCAAGTGGTAACAATGGAAGATGCCTCACATATCGGTCAGGTGTTCGTTACGACCACGGGTTGTCGTGATATCATCAACGGGGAGCATTTCATTAACATGCCGGAAGATGCAATTGTTTGTAACATCGGGCATTTCGATATTGAGATCGACGTGGCGTGGCTCAAAGCCAATGCCAAAGAGTGTATTAACATTAAACCGCAGGTAGATCGTTATTTGCTGAGCTCTGGCCGTCATGTCATCCTCCTGGCAAATGGACGCCTTGTGAATCTGGGTTGTGCGACGGGTCACTCGTCCTTCGTCATGAGCTGCTCTTTTTCCAACCAGGTTCTGGCACAAATCGCCCTGTTTAAATCTAATGACAAATCTTTTCGCGAAAAACACATCGAGTTCCAGAAGACCGGTCCCTTCGAAGTCGGGGTTCATGTGTTGCCTAAAATCTTAGATGAGGCAGTCGCTAAATTTCATCTTGGCAACCTGGGTGTCCGTCTGACCAAACTGAGTAAAGTCCAGAGCGAGTACCTGGGTATTCCGGAGGAAGGACCTTTCAAAGCGGATCATTATCGCTATTAA GCGGCCGCAAATTT