Fig. 5.

Maximum-likelihood phylogeny of the COI gene sequences for selected specimens of the 14 collected mosquito species detected in the Danube Delta Biosphere Reserve (Romania) during the sampling period in 2014 (red font) and additional sequences retrieved from the NCBI nucleotide database (http://www.ncbi.nlm.nih.gov). Red marked sections of the tree and the magnified areas in grey/orange indicate the location of the mosquito species detected in this study. The clades including Aedes hungaricus and Anopheles algeriensis (first reports for Romania) are highlighted in orange. The maximum likelihood bootstrap replicates (≥70 %) and parallel NJ bootstrap values above 70 (1000 replicates) are indicated with an asterisk at the nodes. The scale-bar indicates the genetic distance scale expressed as mean number of nucleotide substitutions per site