Fig. 1.

Overexpression of dnaB inhibits growth of cells lacking the accessory replicative helicase Rep. Induction of dnaB overexpression was performed using a plasmid-based, arabinose-inducible promoter system. Strains harbouring the indicated plasmids were grown in the absence of arabinose and then serial dilutions plated onto LB agar without and with 0.2% arabinose. Wild type, rep, uvrD and priC strains (TB28, N6577, N6632 and MKG3, respectively) were transformed with pBAD or pBADdnaB (pJGB143).