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Overexpression of dnaB perturbs chromosome replication in wild type and in Δrep cells. Strains were grown in LB in the absence of arabinose and then shifted into LB plus 0.2% arabinose for 2 h. Initiation at oriC and cell division were then inhibited for 2 h (“runout conditions”) and DNA content was monitored by flow cytometry. Strains (a) and (b) were TB28 harbouring pBAD and pJGB143, respectively, whilst strains (c) and (d) were N6577 with pBAD and pJGB143.
