Buffer	Composition	Application
Lysis Buffer	10 mM Tris–HCl, pH 7.5	Preparation of lysate (1.1)
	10 mM NaCl
	10 mM MgCl2
	1% Triton-X100
	1% Na-deoxycholate
	0.4 U/ml RNase Inhibitor
	1 mM DTT
	0.2 mg/ml cycloheximide
	5 U/ml Dnase I
50% sucrose solution	50% (w/v) sucrose in	Sucrose gradient preparation (1.2)
	100 mM NaCl
	10 mM MgCl2
	10 mM Tris/HCl pH 7.5
15% sucrose solution	15% (w/v) sucrose in	Sucrose gradient preparation (1.2)
	100 mM NaCl
	10 mM MgCl2
	10 mM Tris/HCl pH 7.5
Nickel Solution	1 mM NiSO4	Sample preparation for AFM (2)
Buffer-AFM	100 mM NaCl	Sample preparation for AFM (2)
	10 mM MgCl2
	100 µg/ml cycloheximide
	10 mM Hepes
	3% (w/v) sucrose, pH = 7.4
Washing Solution	DEPC-Water	Sample preparation for AFM (2)
	100 µg/ml cycloheximide