| Target gene | Primers, probe and plasmide sequences (5'-3') | Nucleotide position | Product size (nucleotides) | Thermal cycling conditions | References | |
| EHV2 gB (HQ247755.1) | Forward: GTGGCCAGCGGGGTGTTC | 2113-2130 | 78 | 95 °C 5 min | 11 | |
| Reverse: CCCCCAAAGGGATTYTTGAA | 2189-2170 | 95 °C 15 sec | 45 cycles | |||
| Probe: FAM-CCCTCTTTGGGAGCATAGTCTCGGGG-MGB | 2132-2157 | 60 °C 1 min | ||||
| Plasmid: ACCTGGGCACCATAGGCAAGGTGGTGGTCA ATGTGGCCAGCGGGGTGTTCTCCCTCTTTG GGAGCATAGTCTCGGGGGTGATAAGCTTTTT CAAAAATCCCTTTGGGGGCATGCTGCTCATA GTCCTCATCATAGCCGGGGTAGTGGTGGTG TACCTGTTTATGACCAGGTCCAGGAGCATAT ACTCTGCCCCCATTAGAATGCTCTACCCCGG GGTGGAGAGGGCGGCCCAGGAGCCGGGCG CGCACCCGGTGTCAGAAGACCAAATCAGGA ACATCCTGATGGGAATGCACCAATTTCAG | 2081-2381 |
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