Name of Material/ Equipment	Quantity (v/v)	Comments/Description
Ham's F-10 Nutrient Mix	40%
DMEM low glucose	40%
KnockOut Serum Replacement	10%
FBS	5%
Antibiotic antimycotic solution	1%
L-Glutamine solution	1%
MEM Non-Essential Amino Acids Solution	1%
2-Mercaptoethanol stock	1%	2-Mercaptoethanol stock preparation: diluite 3.5 µl of 2-Mercaptoethanol in 5 ml of sterile PBS. Store in dark at +4 °C. Note: Use within 2 weeks
Nucleoside mix stock	1%	nucleoside mix stock preparation: dissolve 0.042 g Guanosine, 0.040 g Adenosine, 0.036 g Cytidine, 0.036 g Uridine and 0.012 g Timidine in 50 ml of sterile water. Melt at 50 °C to dissolve. Sterilize by filtration and store at +4 °C
ESGRO (LIF)	0.1%
Recombinant Human FGF basic (bFGF) stock	0.1%	bFGF stock preparation: add 5 ml of 0.1% Bovine Serum Albumin (BSA) in PBS at 25 µg of bFGF