| Step | Temp | Time |
| Initial denaturation | 98 °C | 30 sec |
| 30 cycles | 98 °C | 10 sec |
| 55 °C | 30 sec | |
| 72 °C | 120 sec | |
| Final Extension | 72 °C | 300 sec |
| Storage | 10 °C |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.