Reagent | To Add per Reaction |
Second Round LTR primer (15 µM): | 2.5 µl |
Linker-specific primer (15 µM): | 0.5 µl |
10x PCR buffer: | 2.5 µl |
dNTPs (2.5 mM each) | 0.5 µl |
DNA polymerase mix: | 0.5 µl |
First round PCR: | 100 ng |
Nuclease-free water: | up to 25 µl |