Reagent	To Add per Reaction
Second Round LTR primer (15 µM):	2.5 µl
Linker-specific primer (15 µM):	0.5 µl
10x PCR buffer:	2.5 µl
dNTPs (2.5 mM each)	0.5 µl
DNA polymerase mix:	0.5 µl
First round PCR:	100 ng
Nuclease-free water:	up to 25 µl