Skip to main content
. Author manuscript; available in PMC: 2016 Apr 12.
Published in final edited form as: AIDS. 2008 Nov 30;22(18):2501–2508. doi: 10.1097/QAD.0b013e328318f16c

Fig. 3.

Fig. 3

DNA synthesis reactions carried out by wild-type, TAM67, and TAM67/K65R HIV-1 RT on RNA templates with substitutions at codons 70 (AAA to AGA), 67 (GAC to AAC), 66 (AAA to AAG) and/or 65 (AAA to AAG or AGA).

A) DNA synthesis reactions carried out on the wild-type template as well as RH1, RH3 and RH2.

B) DNA synthesis reactions carried out on the RH1, RH3 and RH4 templates.

C) DNA synthesis reactions carried out by TAM67 HIV-1 RT on the wild-type, RH1, RH3 and RH4 templates.

D) DNA synthesis reactions carried out by TAM67/K65R HIV-1 RT on the wild-type, RH1, RH3, RH4 and RH5 templates.

Reactions were carried out and analyzed as described in Methods. K65K* and K66K* signify the AAA to AAG silent mutations at codons 65 and 66. Times each aliquot ran for, from left to right, are: 1, 2.5, 3.5, 5.5, 7.5, 10, and 20 minutes, respectively.