FIG. 7.

Simultaneous [Ca²⁺]i and AR recordings. A) Fluo-4 AM and FM4-64 images of a representative sperm undergoing AR in which progesterone increases the [Ca²⁺]i in the posterior area of the sperm head and propagates to the anterior zone. The corresponding Fluo-4 AM and FM4-64 fluorescence traces are shown in B. C) Fluo-4 AM and FM4-64 images of a representative example of a cell in which progesterone initiated an [Ca²⁺]i increase in the anterior head zone. The corresponding Fluo-4 AM and FM4-64 fluorescence traces are shown in D. E) Percentage of sperm that underwent AR with anterograde or retrograde propagation (n = 4 mice, 17 reacted cells).