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. 2016 Feb 17;94(3):69. doi: 10.1095/biolreprod.115.134924

FIG. 1.

FIG. 1

Expression levels of DNA damage repair genes RAD51 and BRCA1 in fibroids (F) and adjacent myometrial tissues (MyoF) as well as primary cells. Each experiment was conducted in replicates. A) Expression of RAD51 was determined by real-time PCR in fibroid and adjacent myometrial tissues. *P < 0.05 compared with the respective control (n = 5). B) Expression of BRCA1 was measured by real-time PCR in fibroid and adjacent myometrial tissues. *P < 0.05 compared with the control (n = 5). Expression of RAD51 (C) and BRCA1 (D) was determined by real-time PCR in primary cells from a fibroid and adjacent myometrial tissue. The cells from a fibroid and from myometrium tissue were plates in 6 × 60-mm dishes, including three dishes for primary fibroid cells and three dishes for primary myometrium cells. Three biological samples from both fibroid and myometrium samples were subjected to RNA isolation, cDNA synthesis, and q-PCR analysis. *P < 0.05 compared with the respective control.