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. 2016 Feb 2;94(5):486–495. doi: 10.1038/icb.2016.1

Figure 4.

Figure 4

p110δ is required for T-cell spreading on ICAM-1 and APC. (a) WT and p110δD910A CD4+ T-cell blasts were settled on anti-CD3 mAb plus ICAM-1coated coverslip. DIC and IRM images are shown and quantified. Data in the histogram represent the means of two independent experiments. (b) p110δ is required for T-cell spreading on APC. The polarisation index of WT or p110δD910A CD4+ T-cell blasts interacting with OVA323-339-loaded APC was quantified as described in Methods. A round cell would have a value of 0. Representative images of a conjugate formed by WT or p110δD910A T cells (labelled with a PE-anti-CD3 mAb) are shown. Data are representative of three independent experiments.