Skip to main content
. 2016 Feb 2;94(5):486–495. doi: 10.1038/icb.2016.1

Figure 6.

Figure 6

Inhibition of p110δ disrupts T cell-APC conjugate formation. (a) WT and p110δD910A CD4+ T-cell blasts were loaded onto surfaces coated with ICAM-1 alone or with ICAM-1 and anti-CD3 and imaged by video microscopy for 10 to 15 min. Cell movements were analysed by Volocity software. Data are representative of three independent experiments. (b) WT and p110δD910A OT2 CD4+ T-cell blasts were incubated with OVA323-339-loaded APC for 30 min and assessed for conjugate formation as described in Methods. (c–d) p110δD910A T cells do not slow down as much as the WT when presented antigen by the APC. Data show means of the velocity (c) and arrest coefficient (d) of WT and p110δD910A OT2 CD4+ T-cell blasts during their interaction with APC in presence or absence of OVA323-339 peptide. Data are the mean values of three independent experiments.