Table 3.
Details to prepare and use the FAME mixture as internal standards.
| Chemical | Fiehn retention index value | Kovats retention index value | amount (mg) | concentration (mg/mL) |
|---|---|---|---|---|
| Methyl caprylate/octanoate (C08) | 262320 | 1083 | 20 | 0.8 |
| Methyl pelargonate/nonanoate (C09) | 323120 | 1183 | 20 | 0.8 |
| Methyl caprate/decanoate (C10) | 381020 | 1282 | 20 | 0.8 |
| Methyl laurate/dodecanoate (C12) | 487220 | 1481 | 20 | 0.8 |
| Methyl myristate/tetradecanoate (C14) | 582620 | 1680 | 20 | 0.8 |
| Methyl palmitate/hexadecanoate (C16) | 668720 | 1878 | 20 | 0.8 |
| Methyl stearate/octadecanoate (C18) | 747420 | 2077 | 10 | 0.4 |
| Methyl arachidate/icosanoate (C20) | 819620 | 2276 | 10 | 0.4 |
| Methyl behenate/docosanoate (C22) | 886620 | 2475 | 10 | 0.4 |
| Methyl tetracosanoate (C24) | 948820 | 2674 | 10 | 0.4 |
| Methyl hexacosanoate (C26) | 1006900 | 2872 | 10 | 0.4 |
| Methyl octacosanoate (C28) | 1061700 | 3071 | 10 | 0.4 |
| Methyl triacontanoate (C30) | 1113100 | 3270 | 10 | 0.4 |