Effect of sodium hydrosulfide on apoptotic protein in rats with acute myocardial infarctiom (AMI). (A) Western blot analysis of the indicated proteins. Quantification of (B) Bax/Bcl-2 ratio, (C) Bax and Bcl-2. (D) Quantification of Bax and Bcl-2 immunohistochemical analysis, (E) immunohistochemical analysis of Bax and Bcl-2 (×40 magnification; scale bar, 50 µm). Data are expressed as the means ± SEM. **p<0.01 vs. sham-operated group; #p<0.05 and ##p<0.01 vs. AMI group. Sham, sham-operated group; AMI, rats subjected to AMI (ischemia) and not treated with any agents; AMI + NaHS-L, rats subjected to AMI and treated with low-dose (0.78 mg/kg) NaHS; AMI + NaHS-M, rats subjected to AMI and treated with medium-dose (1.56 mg/kg) NaHS; AMI + NaHS-H, rats subjected to AMI and treated with high-dose (03.12 mg/kg) NaHS; AMI + SB, rats subjected to AMI and treated with the glycogen synthase kinase-3β (GSK-3β) inhibitor, SB216763 (0.6 mg/kg); AMI + vehicle, rats subjected to AMI and treated with the vehicle (DMSO, 2 ml/kg).