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. 2016 Mar 17;37(5):1170–1180. doi: 10.3892/ijmm.2016.2532

Figure 6.

Figure 6

(A) RT-PCR analysis of human RNA in the optic nerves from a rat model of optic nerve crush (ONC) injury after umbilical cord blood mononuclear cell (CB-MNC) or chorionic plate-derived MSC (CP-MSC) transplantation. Equal amounts of RNA were pooled from individual animals of the following groups: non-transplanted group (NTP) at 1 week post-injury, CB-MNC-transplanted (CB-MNC) or CP-MSC-transplanted group (CP-MSC) at 1 week, and CB-MNC-transplanted or CP-MSC-transplanted group at 2 weeks post-transplantation. A human RNA signal was detected 1 week post-CP-MSC injection (red asterisk). Actin was used as a loading control. Kidney samples from the rats in the NTP group and CP-MSC-transplatned group were used as the negative and positive controls, respectively (side panel, bottom right). (B) SRGAP2, NF, ERMN, and OCM levels were measured by western blot analysis using pooled protein samples from individual animals of the NTP group and CP-MSC-transplanted group at 2 weeks post-transplantation. GAPDH was used as loading control. Rt, right eye; Lt, left eye-optic nerve compression side; ERMN, ERM-like protein; LRAT, lecithin-retinol acyltransferase; SRGAP2, SLIT-ROBO Rho GTPase activating protein 2.