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. 2016 Mar 22;37(5):1209–1220. doi: 10.3892/ijmm.2016.2536

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Copyright: © Park et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Schematic of myogenic differentiation from human tonsil-derived mesenchymal stem cells (T-MSCs). (A) The undifferentiated T-MSCs were induced to form (B) spheres of approximately 50–100 µm in diameter on a Petri dish. (C) The T-MSCs grew out of the sphere when transferred to the collagen-coated dish in the replating medium and formed a rosette-like spread. (D) The plated cells were cultured for 2 weeks in myogenic induction medium and their morphology was altered; they underwent fusion with one another to generate nascent myotubes. Original magnification, ×40. lgDMEM, low-glucose DMEM; NEAA, non-essential amino acids; ITS, insulin-transferrin-selenium.