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. 2016 Mar 24;37(5):1189–1198. doi: 10.3892/ijmm.2016.2537

Figure 6.

Figure 6

High-mobility group box 1 protein (HMGB1) in synergy with interleukin (IL)-1β enhances epithelial barrier dysfunction. 16HBE cells were stimulated with 100 ng/ml HMGB1 in the presence or absence of 2.5 ng/ml IL-1β for 24 h. (A) Transepithelial electrical resistance (TER) and (B) FITC-dextran permeability were measured after 24-h exposure to the indicated treatments (n=6 experiments). (C) E-cadherin, β-catenin, occludin and claudin-1 were detected by western blot analysis. The delocalization of (D) E-cadherin and (E) β-catenin was shown by immunofluorescence staining. Representatives of 3 independent experiments are shown. The levels of TER were normalized to those prior to HMGB1 or medium (control) stimulation, and the levels of FITC-dextran permeability were normalized to the ratio between HMGB1 and medium only (control) stimulation, which was denoted by Pa/Pc. P-values were calculated using an ANOVA test. *P<0.05, vs. stimulation with 100 ng/ml HMGB1; scale bar, 50 mm.