Skip to main content
. 2016 Apr 12;11(4):e0150255. doi: 10.1371/journal.pone.0150255

Fig 2. Nitric oxide availability and eNOS expression in ACE2-/y mice.

Fig 2

Plasma nitrite content (A) and 24-hour urinary excretion (B) of nitrate plus nitrite (NOx) in ACE2-/y (n = 6) and WT (n = 6) mice. (C) eNOS activity, evaluated by •NO release in aorta rings upon application of ACh (DAF-FM bioassay) (ACE2 n = 5; WT n = 6). (D) arginase activity in aorta homogenates of ACE2-/y (n = 6) and WT (n = 6) mice. Representative western blot and densitometric analysis of eNOS (E) and phosphorylated Ser1177-eNOS (F) protein levels in thoracic aortas from ACE2-/y (n = 5) and WT (n = 5) mice. (G) Relative expression of phospho-eNOS normalized to the total eNOS levels. (H) eNOS mRNA expression levels (real time PCR analysis). Data are expressed as mean ± SEM. *P<0.05, **P<0.01, **P<0.001, Student’s t test.