Body weight, self-righting reflex, eye opening was checked to determine if VPA mice show behavioral developmental delays, while maternal scent preference was conducted to confirm social recognition impairments. (A) VPA mice showed significantly decreased body weight from on P5-9. (B) VPA mice showed increased latency to self-right on P7-9. (C) VPA mice showed lower eye opening index scores on P13. (D) SAL mice spent more time in the zone with familiar scent, while VPA mice did not show difference in time spent in each zone. (A-C, * significantly different between SAL and VPA mice on same date, * p < 0.05, ** p < 0.01. D, * significantly different between zones, *** p < 0.001. n = 9 for SAL, n = 10 for VPA in all experiments.)