Fig 1. Generation of the Kif13b-null mouse in Schwann cells.

(A) Kif13b protein domains. (B) The Kif13b targeting vector and the recombined allele following deletion of exon 6. Thick red lines represent genomic sequences, and thin black lines correspond to the pFlrt1 vector used for homologous recombination. (C) Southern blot analysis performed to identify ES cell recombinant clones. (D) PCR analysis using primers A and C performed on DNA from tail and sciatic nerve of wild type (+/+) and Kif13b ^Fl/Fl P0-Cre mice. (E) Western blot analysis on lysates from mouse sciatic nerves and isolated rat Schwann cells shows reduction of Kif13b expression in the mutant.