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. 2016 Apr 12;11(4):e0153130. doi: 10.1371/journal.pone.0153130

Fig 1. Quantitative real-time PCR validation of differentially expressed mature miRNAs.

Fig 1

Total small RNA fractions were prepared from F28-7 and F28-7-A cells (no drug, no incubation). Expression of (A) miR-700, (B) miR-743a, (C) miR-140*, (D) miR-351, (E) miR-222, (F) miR-34c*, (G) miR-132, and RNU6B were analyzed by quantitative real-time PCR using primers for miR-700-3p, miR-743a-3p, miR-140-3p, miR-351-5p, miR-222-3p, miR-34c-3p, miR-132-3p, and RNU6B (see Materials and Methods). The expression of RNU6B was used as an internal control. Results are averages of three independent experiments with error bars showing the ±SD in triplicates. The asterisk indicates a statistically significant difference (Student’s t test, *p<0.05, **p<0.01).