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. 2016 Apr 12;11(4):e0153130. doi: 10.1371/journal.pone.0153130

Fig 3. Higher expression of miR-351 shifts the FUdR-induced cell death morphologies from necrosis to apoptosis.

Fig 3

(A) At 48 hours after transfection with the vehicle, non-silencing siRNA (NSsi) or the mature miR-351-5p mimic (miR351m), the F28-7 cells were treated with or without 1 μM FUdR for 21 h, and then stained with DAPI. Morphological changes were analyzed by Olympus BX61 fluorescence microscope at 400× magnification. (B) Percentage of necrosis, apoptosis, and normal cell morphologies in FUdR-treated F28-7 cells transfected with NSsi or miR351m. (C) At 48 hours after transfection with the vehicle, non-silencing siRNA (NSsi) or the miR-351 mimic (miR351m), the F28-7 cells were treated with or without 1 μM FUdR for 21 h, and analyzed by western blotting for anti-HMGB1 antibody. As a negative control, F28-7-A cells were treated with or without 1 μM FUdR for 21 h, and examined by western blotting. Two additional independent experiments gave similar results.