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. 2016 Apr 12;12(4):e1005570. doi: 10.1371/journal.ppat.1005570

Fig 3. ToxR negatively regulates VSP-1 encoded genes affecting host colonization.

Fig 3

(A) qRT-PCR analysis of VC0176, VC0178, and VC0493 gene expression. The expression level of these genes in the ΔtoxRS+ptoxRS strain is shown, normalized to expression levels in the ΔtoxRS+vector control strain, which was set at 1. VC0176, VC0178, and VC0493 gene expression is decreased in ΔtoxRS+ptoxRS compared to the control. ****p < 0.0001, unpaired two-tailed Student’s t test. (B) Competition assays of ΔVC0176 mutant vs. wild type in vitro and in vivo in the infant mouse model. Each point represents an individual mouse result. ΔVC0176 has a colonization defect vs. wild type in vivo in the infant mouse model compared to in vitro. ****p < 0.0001, unpaired two-tailed Student’s t test.