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. 2016 Apr 12;12(4):e1005570. doi: 10.1371/journal.ppat.1005570

Fig 5. TcpP directly regulates toxT, ompT, and VCA0536.

Fig 5

(A) TcpP ChIP enrichment of the promoter regions of toxT, ompT, and VCA0536. Enrichment of a non-TcpP-dependent promoter icd is shown as a control. TcpP enrichment of the promoter regions of toxT, ompT, and VCA0536 is statistically significant compared to the control. **p < 0.01; *p < 0.05, unpaired two-tailed Student’s t test. (B) qRT-PCR analysis of ompT and VCA0536 gene expression. The expression level of these genes in the ΔtcpPH+ptcpPH strain is shown, normalized to expression levels in the ΔtcpPH+vector control strain, which was set at 1. ompT expression is decreased in ΔtcpPH+ptcpPH compared to the control strain, while VCA0536 expression is increased in ΔtcpPH+ptcpPH compared to the control strain. ****p < 0.0001; **p < 0.005, unpaired two-tailed Student’s t test. Mean with standard error of the mean (SEM) is shown.