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. Author manuscript; available in PMC: 2016 Apr 12.
Published in final edited form as: Chromosome Res. 2013 Sep 24;21(8):765–779. doi: 10.1007/s10577-013-9381-9

Fig. 1.

Fig. 1

Emerin binds to myogenic regulatory factor loci and regulates their association with the nuclear lamina. a ChIP-qPCR of MyoD and Myf5 promoters with antibodies against emerin or IgG in wildtype proliferating myogenic progenitors. Fold-enrichment was determined by comparing emerin to IgG. Error bars represent S.E.M. n=3. b 3D-ImmunoFISH showing the localization of Myf5 in proliferating wildtype and emerin-null myogenic progenitors. Lamin B1 staining is red and Myf5 is green. c Quantification of localization data for Myf5 in proliferating myogenic progenitors. n=50. Peripheral is ≤1 μm from the nuclear lamina. Intermediate is between 1 μm and 2 μm from the nuclear lamina. Central is ≥2 μm from the nuclear lamina. d 3D-ImmunoFISH showing the localization of MyoD in proliferating wildtype and emerin-null myogenic progenitors. Lamin B1 staining is red and MyoD is green. e Quantification of localization data for MyoD in proliferating myogenic progenitors. n=50. Peripheral is ≤1 μm from the nuclear lamina. Intermediate is between 1 μm and 2 μm from the nuclear lamina. Central is ≥2 μm from the nuclear lamina. Scale bars are 5 μm.