Fig. 3.

Emerin regulates changes in genomic architecture during myogenic differentiation. a 3D-ImmunoFISH of Pax7 localization in proliferating and differentiating wildtype and emerin-null myogenic progenitors. Lamin B1 is red, Pax7 is green. Scale bars are 5 μm. b Quantification of localization data for Pax7. p<0.05 when comparing wildtype localization at differentiation day 1 to proliferating cells or emerin-null differentiation day 1; n=50. Peripheral is ≤1 μm from the nuclear lamina. Intermediate is between 1 μm and 2 μm from the nuclear lamina. Central is ≥2 μm from the nuclear lamina. (c) 3D-ImmunoFISH of MyoD and Myf5 localization in wildtype and emerin-null myogenic progenitors at differentiation day 1. Lamin B1 is red and MyoD or Myf5 is green. Scale bars are 5 μm. d Quantification of localization data for MyoD and Myf5 in wildtype and emerin-null myogenic progenitors at day 1 of differentiation. Peripheral is ≤1 μm from the nuclear lamina. Intermediate is between 1 μm and 2 μm from the nuclear lamina. Central is ≥2 μm from the nuclear lamina. e qPCR of Myf5 and Pax7 mRNA in emerin-null proliferating or differentiation day 1 myogenic progenitors relative to wildtype proliferating or differentiation day 1 myogenic progenitors, respectively, normalized to GAPDH. Error bars represent S. E. M. n=3. f Percentage of MyoD, Myf5, Pax3, and Pax7 loci in wildtype myogenic progenitors within 1 μm of the nuclear lamina throughout differentiation. Dotted line represents the percentage of loci expected within 1 μm of the nuclear lamina by chance. g Percentage of MyoD, Myf5, Pax3, and Pax7 loci in emerin-null myogenic progenitors within 1 μm of nuclear lamina throughout differentiation. Dotted line represents percentage of loci expected within 1 μm of the nuclear lamina by chance