Fig. 5.
Stimulation of HDAC3 activity restores association of Myf5 with the nuclear lamina and reduces Myf5 mRNA expression. a 3D-ImmunoFISH analysis of emerin-null proliferating myogenic progenitors treated with vehicle (H2O). Lamin B1 is red, Myf5 is green. b 3D-ImmunoFISH of emerin-null proliferating myogenic progenitors treated with 10 μM theophylline. Lamin B1 is red, Myf5 is green. c Quantification of Myf5 localization in vehicle and theophylline-treated emerin-null proliferating myogenic progenitors. n=50. p<0.05. Peripheral is ≤1 μm from the nuclear lamina. Intermediate is between 1 μm and 2 μm from the nuclear lamina. Central is ≥2 μm from the nuclear lamina. d 3D-ImmunoFISH of wildtype proliferating myogenic progenitors treated with vehicle (H2O). Lamin B1 is red, Myf5 is green. e 3D-ImmunoFISH of wildtype proliferating myogenic progenitors treated with 10 μM theophylline. Lamin B1 is red, Myf5 is green. f Quantification of Myf5 localization in vehicle and theophylline-treated wildtype proliferating myogenic progenitors. n=50. Scale bars are 5 μm. g qPCR of Myf5 mRNA in wildtype and emerin-null cells treated with 10 μM theophylline for 4 h relative to mock treatment. Expression was normalized to GAPDH. Error bars represent S.E.M. n=3