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. 2016 Apr 12;12(4):e1005565. doi: 10.1371/journal.ppat.1005565

Fig 8. Full Length HIV-1 and M-PMV gRNA exhibit burst nuclear export and MTOC-targeting phenotypes, respectively.

Fig 8

(A) Representative images of full-length HIV-1 and M-PMV reporter viruses modified to carry the 24xMBL cassette and express Gag-CFP (blue) in HeLa.MS2-YFP cells at 24 hours post-transfection. Dotted line indicates nuclear/cytoplasmic boundary after gRNA (green) was evacuated from the nucleus. Black and yellow arrows indicate the location of the centrosomes based on Pericentrin (red) immunofluorescence. (B) MTOC-targeting for HIV-1 and M-PMV gRNAs as measured for Fig 4E. (C) M-PMV gRNAs accumulated at the MTOC in both human HeLa (top panels) and African green monkey Cos7 cells (bottom panels). (D) M-PMV gRNA clustering at the MTOC precedes Gag-CFP synthesis. Gag-CFP synchronized rise in mean fluorescence intensity (MFI) over time (lower panel, n = 10 cells) relative to the defined subcellular distribution of MS2-YFP for the same cells (top panel). All size bars represent 10 μm.